Claudio Santos

Claudio Santos

Claudio Santos

I completed my UG degree in Chemistry and went on to study an MSc in Biochemistry in 2008 in my home country of Portugal. I was involved in a variety of interdisciplinary projects which developed by skills and interest in nuclear magnetic resonance (NMR) technology, metabolomics and molecular biology.

My desire to improve my knowledge in this area whilst improving my English led me to apply for a PhD in the UK. I wanted to study at an international institute with a reputation for pushing the boundaries in technology development and innovation. I was delighted to join the Manchester Institute of Biotechnology as it promotes interdisciplinary, challenge oriented science that is supported by an outstanding structural biology infrastructure.

The main goal of my PhD project is to develop a novel NMR-based assay capable of measuring 13C competitive kinetic isotope effects (KIEs) on enzymatic methyl transfer reactions. The model system that we will use is catechol-O-methyl transferase (COMT) and we want to measure KIEs on methyl transfer in COMT as a means of probing the reaction mechanism. The NMR intensity of methyl groups will be measured and refined. With this in place, this methodology will be used to measure the pressure and temperature dependence of the COMT reaction with a range of substrates to search for evidence of C-tunneling.

The city of Manchester is notable for its architecture, culture, music scene, media links, scientific and engineering output, social impact and sporting connections.

“The MIB has a reputation for pushing the boundaries in technology development and innovation. I was delighted to join the Manchester Institute of Biotechnology as it promotes interdisciplinary, challenge oriented science that is supported by an outstanding structural biology infrastructure.”