NMR Facility
The Facility consists of a DPX400MHz Bruker instrument, set up for open - access and automation, and a 600MHz research instrument. Both housed in the purpose built NMR suite.Pulsed Fourier Transform Nuclear Magnetic Resonance spectroscopy (FT-NMR) is one of the most powerful and versatile non-destructive spectroscopic methods for structure elucidation.The types of information accessible via high resolution NMR include;
- Functional group analysis (chemical shifts)
- Bonding connectivity and orientation (J coupling),
- Through space connectivity (Overhauser effect)
- Molecular Conformations, DNA, peptide and enzyme sequence and structure.
- Chemical dynamics (Lineshapes, relaxation phenomena).
The most important applications for the organic chemist are proton, 1H NMR and carbon-13, 13C NMR spectroscopy. In principle, NMR is applicable to any nucleus possessing spin, such as 19F and 31P.
Many types of information can be obtained from an NMR spectrum. Analysis of a 1-dimensional (1D) NMR spectrum provides information on the number and type of protons and carbon atoms in a molecule. Further pulse programs such as COSY can reveal their bonding connectivity and orientation. A very common application is NOESY (nuclear overhauser effect spectroscopy), an NMR technique used to display “through space” connectivity often used to aid in the structural determination of macromolecules i.e. proteins.
There are a range of other pulse programs available, tailored to the specific requirements of the analyst, such as; DEPT, TOCSY, HSQC, HMBC and many others.
For a description of these programs or to discuss your analysis needs, please contact:
Paul Williams on 0161 306 5185; paul.williams-2@manchester.ac.uk