This facility includes instrumentation for the imaging, manipulation or measurement of single biomolecules or single molecule biochemical reactions.
Bionanotechnology techniques allow:
- Imaging of single molecules in air or in buffer solution.
- Unbeatable sensitivity and specificity when detecting molecules and molecular interactions in solution or on a surface.
- Gathering of quantitative data on discrete systems for input into systems biology models.
- Identification of rare alternative reaction pathways and intermediates that are normally masked by ensemble averaging.
- Investigation of the mechanics of biological polymers and cell membranes.
Recent AFM projects include imaging of graphene on silicon oxide in air, fixed xenopus embryos in PBS, protein fibrils, mucins, gold nanoparticles, etched troughs and pits in silicon, end to end protein stretching and a selection of cell and surface indentation experiments on the JPK CellHesion AFM.
- Multimode-Picoforce Atomic Force Microscope (AFM)
- JPK Cellhesion AFM
- Total Internal Reflection Fluourescence Microscope (TIRFM)
- Raman Laser Tweezers
- Fluorescence Correlation Spectrometer (FCS)
- Typhoon Trio+ Variable Mode Imaging System for scanning fluorescent and radiolabelled gels
- Olympus BX51 upright fluorescence snapshot microscope with colour and monochrome cameras.
The facility has been set up to promote and facilitate the use of bionanotechnology techniques mainly for the study of single molecule imaging. We provide full training for new users to ensure safe and proper use of all instrumentation and can provide further support and instruction where required.
In addition a large selection of widefield, confocal and specialist microscopes are available in the Michael Smith Building Bioimaging Facility.